![]() ![]() Β-actin is a housekeeping gene and an obligatory part of the cell cytoskeleton. Examples of housekeeping genes include actin, GAPDH and ubiquitin. The proteins they code are generally involved in the basic functions necessary for the sustenance or maintenance of the cell. Loading controls are typically proteins with high and ubiquitous expression. Loading controls also indicate the proper transfer of proteins to the membrane during the western blotting process. ![]() Loading control antibodies are important controls as they indicate the equal loading of samples across all wells. What is the purpose of a loading control? When used correctly, loading controls ensure that proteins are quantitated appropriately despite subtle differences in loading amounts across all lanes of the Western blot. Loading controls can be used to show whether protein loading variation has occurred and may account for observed variations in the target band(s). The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control.Nov 9, 2011 Loading control antibodies help in assessing that samples have been loaded equally across a gel.īeta-Actin (42 kDa) is commonly chosen as a loading control due to its general expression across all eukaryotic cell types. Loading Control: A loading control is an antibody specific for a ubiquitously and constitutively expressed protein and can be used to normalize protein levels in Western blot. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.Beta-actin is also present in the nucleus, as a component of chromatin remodeling complexes, but it can not be used as a control for nuclear protein samples. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. An important paralog of this gene is ACTG1. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Special Offer: Custom Recombinant Antibody Production ServiceĪCTB (Actin Beta) is a Protein Coding gene.Free Sample of Premium Quality Cytokines and Growth Factors.Producing Challenging Proteins in the Golden Age of Protein Engineering.Sino Biological Europe: A New Face in the European Life Science Market. ![]() The Evolution of Recombinant Antibodies.Industry Insights with Yuning Chen on Recombinant Proteins.ExpertAnswers: Yuning Chen on Antibody Production.Universal Vaccine Advancement through AI and Recombinant Technology.Common Cytokine Receptor Signaling Pathway. ![]() SARS-CoV-2 Variant Detection Antibodies.Immunodetection for Pan Influenza NP Antigens.SARS-CoV-2 Prefusion Trimeric Spike Variant. ![]()
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